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Basic Neuroscience · Neuropathology

Neuro-oncology Pathology

CNS tumor pathology — pathognomonic histological findings, then each tumor's clinical picture, imaging, and biopsy appearance. The downloadable PDF includes the corresponding stained histology slides.

Pathognomonic findings

Pathological findingTumor
Perivascular pseudorosetteEpendymoma, SEGA
Rosenthal fibersPilocytic astrocytoma
Rosettes (Homer-Wright)Medulloblastoma
Wet keratinCraniopharyngioma
Psammoma bodiesMeningioma
"Fried egg" cellsOligodendroglioma

CNS tumors

TumorClinical & imagingPathology
MedulloblastomaChildren; midline cerebellum; diffuse contrast enhancement. Can seed in CSF ("drop mets") but rarely involves meninges. MRS shows choline and taurine peaks.Stains positive for synaptophysin; rosette formation.
Pilocytic astrocytomaChildren; cystic with an enhancing mural nodule. Can occur in the optic tract in NF1. Associated with BRAF mutation.Cells with long processes; Rosenthal fibers (intense red deposits of hyaline). Hair-like processes arranged in bundles resembling mats of hair.
SEGAIn tuberous sclerosis (TSC1 on ch 9q34, TSC2 on ch 16).Large polygonal cells with abundant eosinophilic cytoplasm; perivascular pseudorosettes.
OligodendrogliomaAdults; lobar; associated with IDH mutation. Anaplastic (grade III) associated with allelic loss at 1p and 19q.Rounded nuclei, prominent cytoplasm with a clear halo ("fried egg").
Colloid cystUsually arises in the 3rd ventricle near the foramen of Monro. MRI: isointense on T1, hyperintense on T2.Simple cuboidal or columnar epithelium, full of proteinaceous material.
EpendymomaUsually arises in the 4th ventricle (children) or spinal cord (adults). Can seed through CSF.Perivascular pseudorosettes — ependymal cell processes directed toward the vessel wall, with anuclear zones of GFAP+ fibrillary processes.
SubependymomaArises in the 4th ventricle in adults; does not enhance.Clusters of cells embedded in a dense glial fibrillary background; pseudorosettes may be present.
HemangioblastomaCystic cerebellar tumor in adults with a nodule of blood vessels; can involve the spine in VHL.Vacuolated cells and vascular structures — packed thin-walled vessels and large neoplastic cells with pink-to-clear cytoplasm containing fine PAS+ lipid vacuoles.
MeningiomaExtra-axial dural-based mass.Psammoma bodies (laminated calcific concretions) and whorl formation (cells arranged in whorls).
Choroid plexus papillomaArises in the lateral ventricle in children; homogeneous enhancement.Papillary or villous architecture — a single layer of epithelial cells overlying a fibrovascular core.
Pituitary adenomaSellar mass.Rounded or polygonal cells with rounded nuclei; cytoplasm chromophobic, acidophilic, or basophilic according to hormone production.
CraniopharyngiomaSuprasellar tumor in children; usually calcified.Keratinocytes in spheres ("wet keratin").
Rathke's cleft cystSuprasellar cystic tumor; isointense, non-enhancing.Cyst lined with ciliated columnar epithelium with goblet cells.
Hypothalamic hamartomaIn children; causes gelastic seizures and precocious puberty. MRI: non-enhancing, isointense.Hypocellular mass of mature glia and neurons.
GerminomaSuprasellar or pineal (most common pineal tumor).Small reactive lymphocytes and large neoplastic germ cells.
PineocytomaAdults; low-grade tumor; contrast enhancing.Large anuclear areas called pineocytomatous rosettes.
DNETChildren or young adults. MRI: cortical, "soap bubble" appearance, no enhancement.Glioneuronal tumor with a specific glioneuronal element.
CNS lymphomaUsually arises at the edge of the ventricle. MRI: homogeneous enhancement, vasogenic edema on T2, diffusion restriction on DWI.Angiocentric proliferation of atypical lymphoid cells.
Epidermoid cystCaused by entrapment of ectodermal tissue in the neural tube during development. MRI: CSF signal on T1/T2, heterogeneous on FLAIR, restricted diffusion (unlike arachnoid cyst).Cyst lined by squamous epithelium, filled with keratin and cholesterol.

LIGHTS on RITE by Ahmed Koriesh