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TRAILBLAZER-ALZ 2

Donanemab in Early Symptomatic Alzheimer Disease: The TRAILBLAZER-ALZ 2 Randomized Clinical Trial

Year of Publication: 2023

Authors: John R. Sims, Jennifer A. Zimmer, Cynthia D. Evans, ..., Daniel M. Skovronsky

Journal: JAMA

Citation: JAMA. 2023;330(6):512-527

Link: https://doi.org/10.1001/jama.2023.13239


Clinical Question

Does donanemab, a monoclonal antibody designed to clear brain amyloid plaque, provide clinical benefit in early symptomatic Alzheimer disease?

Bottom Line

Donanemab significantly slowed clinical progression at 76 weeks in participants with early symptomatic Alzheimer disease and amyloid and tau pathology, with 35% slowing in low/medium tau and 22% in combined population, though ARIA occurred in 24% of treated patients with 3 treatment-related deaths

Major Points

  • Phase 3 trial enrolling 1736 participants with early symptomatic Alzheimer disease stratified by tau pathology (low/medium vs high)
  • Primary outcome met: iADRS showed 35.1% slowing in low/medium tau population (difference 3.25 points, p<0.001)
  • CDR-SB showed 36% slowing in low/medium tau population (difference -0.67, p<0.001)
  • 80% of low/medium tau participants achieved amyloid clearance (<24.1 Centiloids) at 76 weeks
  • Limited-duration dosing design: participants switched to placebo upon achieving amyloid clearance (52% completed by 1 year)
  • High tau population showed smaller benefits (non-significant for iADRS, significant for CDR-SB)
  • 38.6% lower risk of disease progression on CDR-G with donanemab treatment
  • ARIA-E occurred in 24% of donanemab group (6.1% symptomatic); 3 treatment-related deaths after serious ARIA
  • Plasma P-tau217 significantly reduced with donanemab treatment

Design

Study Type: Randomized, double-blind, placebo-controlled phase 3 trial

Randomization: 1

Blinding: Double-blind; participants and investigators blinded; CDR raters blinded to adverse event information

Enrollment Period: June 2020 to November 2021

Follow-up Duration: 76 weeks

Centers: 277

Countries: United States, Japan, Canada, United Kingdom, Spain, Netherlands, Australia, Puerto Rico

Sample Size: 1736

Analysis: NCS2 (natural cubic spline with 2 degrees of freedom) for iADRS; MMRM for CDR-SB; intention-to-treat; gated testing scheme with α=0.04 for low/medium tau and α=0.01 for combined population


Inclusion Criteria

  • Age 60-85 years
  • Early symptomatic Alzheimer disease (MCI or mild dementia)
  • MMSE score 20-28
  • Amyloid pathology ≥37 Centiloids on 18F-florbetapir or 18F-florbetaben PET
  • Tau pathology (low/medium or high) on 18F-flortaucipir PET

Exclusion Criteria

  • Presence of amyloid-related imaging abnormalities of edema/effusion on MRI
  • More than 4 cerebral microhemorrhages
  • More than 1 area of superficial siderosis
  • Any intracerebral hemorrhage >1 cm
  • Severe white matter disease on MRI

Arms

FieldDonanemabControl
InterventionDonanemab 700mg IV for first 3 doses, then 1400mg IV every 4 weeks for up to 72 weeks; switched to placebo upon achieving amyloid clearance (<11 Centiloids on single scan or <25 on 2 consecutive scans)Matching placebo IV every 4 weeks
DurationUp to 72 weeks76 weeks

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Change in integrated Alzheimer Disease Rating Scale (iADRS) score from baseline to 76 weeks (range 0-144, lower scores indicate greater impairment)Primary<0.001
CDR-SB change at 76 weeks (Low/Medium Tau)Secondary1.881.20<0.001
iADRS change at 76 weeks (Combined Population)Secondary-13.11-10.19<0.001
CDR-SB change at 76 weeks (Combined Population)Secondary2.421.72<0.001
ADAS-Cog13 change at 76 weeks (Low/Medium Tau)Secondary4.693.17<0.001
ADCS-iADL change at 76 weeks (Low/Medium Tau)Secondary-4.59-2.76<0.001
Amyloid clearance at 76 weeks (Low/Medium Tau)Secondary0%80.1%<0.001
CDR-G progression risk (Low/Medium Tau)SecondaryReference38.6% lower riskHR 0.61 (95% CI 0.47-0.80)<0.001
Plasma P-tau217 log10 change (Low/Medium Tau)SecondaryReference-0.25 difference<0.001
DeathAdverse10 (1.1%)16 (1.9%)
Treatment-related deathAdverse1 (0.1%)3 (0.4%)
Serious adverse eventsAdverse138 (15.8%)148 (17.4%)
ARIA-E (any)Adverse18 (2.1%)205 (24.0%)
ARIA-E (symptomatic)Adverse1 (0.1%)52 (6.1%)
ARIA-E (serious)Adverse063 (7.5%)
ARIA-H (microhemorrhage or siderosis)Adverse119 (13.6%)268 (31.4%)
Infusion-related reactionAdverse4 (0.5%)74 (8.7%)
Intracerebral hemorrhage >1cmAdverse2 (0.2%)3 (0.4%)

Subgroup Analysis

Benefits were generally consistent across baseline characteristic subgroups. Post hoc analysis of high tau population alone showed non-significant difference on iADRS (difference 1.26, p=0.42) but significant difference on CDR-SB (difference -0.69, p=0.006). ARIA-E rates by APOE ε4 status: noncarrier 15.7%, heterozygote 22.8%, homozygote 40.6%.


Criticisms

  • Limited racial/ethnic diversity (91.5% White participants)
  • Variable donanemab dosing due to limited-duration design creates interpretation challenges
  • High tau population showed attenuated benefits, limiting generalizability
  • Three treatment-related deaths occurred after serious ARIA events
  • Conducted during COVID-19 pandemic which was most common adverse event
  • APOE ε4 carriers excluded from subset of analyses despite being majority of population
  • Cannot directly compare to other amyloid-targeting trials due to design differences (tau stratification)
  • Possible unblinding due to ARIA and infusion reactions

Funding

Eli Lilly and Company

Based on: TRAILBLAZER-ALZ 2 (JAMA, 2023)

Authors: John R. Sims, Jennifer A. Zimmer, Cynthia D. Evans, ..., Daniel M. Skovronsky

Citation: JAMA. 2023;330(6):512-527

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