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AHEAD 3–45

The AHEAD 3-45 Study: Design of a Prevention Trial for Alzheimer's Disease (BAN2401-G000-303)

Year of Publication: 2023

Authors: Michael S. Rafii, Reisa A. Sperling, Michael C. Donohue, ..., Paul S. Aisen

Journal: Alzheimer's & Dementia

Citation: Alzheimers Dement. 2023 April; 19(4): 1227-1233

Link: https://doi.org/10.1002/alz.12748


Clinical Question

Can intervention with lecanemab (BAN2401), initiated during the asymptomatic/preclinical stage of AD, slow biomarker changes (amyloid/tau accumulation) and/or cognitive decline in cognitively unimpaired individuals?

Bottom Line

Design paper for ongoing trial - no results available. The AHEAD 3-45 Study is testing whether early intervention with lecanemab can prevent or slow AD progression in two populations: early preclinical AD (intermediate amyloid) and preclinical AD (elevated amyloid).

Major Points

  • Launched July 14, 2020 as a public-private partnership between ACTC, NIA/NIH, and Eisai Inc.
  • Two sister trials under single protocol: A3 (intermediate amyloid, Phase 2) and A45 (elevated amyloid, Phase 3)
  • A45 Trial: ~1000 participants with elevated amyloid (>40 Centiloids), cognitive primary endpoint (PACC5)
  • A3 Trial: ~400 participants with intermediate amyloid (20-40 Centiloids), PET imaging primary endpoint
  • Treatment duration 216 weeks (4+ years) with 12-week follow-up after last infusion
  • Innovative plasma biomarker screening using C2N mass spectrometry Aβ42/40 ratio to reduce unnecessary PET scans
  • Recruitment enriched via TRC-PAD, ADNeT Registry, and J-TRC trial-ready cohorts
  • Uses NAV4694 (amyloid) and MK6240 (tau) PET tracers for optimal sensitivity

Design

Study Type: Phase 2 (A3) and Phase 3 (A45) randomized controlled trials under single master protocol

Randomization: 1

Blinding: Double-blind; all participants, investigators, site personnel, ACTC coordinating center, and sponsor staff masked; unblinded pharmacist prepares infusions; two medical monitoring teams (blinded and unblinded) for safety oversight

Enrollment Period: Launched July 14, 2020; ongoing

Follow-up Duration: 216 weeks treatment + 12 weeks follow-up

Countries: United States, Australia, Japan

Sample Size: 1400

Analysis: MMRM for primary analysis; includes baseline value, age, treatment arm, APOE ε4 status, sex, education, geographical region, visit, baseline-by-visit interaction, and treatment arm-by-visit interaction as fixed effects; A3 and A45 data analyzed separately


Inclusion Criteria

  • Age 55-80 years
  • Cognitively unimpaired
  • A3 Trial: Intermediate brain amyloid (≈20-40 Centiloids) on screening amyloid PET
  • A45 Trial: Elevated brain amyloid (>40 Centiloids) on screening amyloid PET
  • On the 'Alzheimer's continuum' per NIA-AA Research Framework

Exclusion Criteria

  • Not explicitly listed in design paper

Arms

FieldA45 LecanemabControlA3 LecanemabControl
InterventionLecanemab 5 mg/kg IV Q2W for 6 weeks (titration), then 10 mg/kg IV Q2W for 86 weeks (induction), then 10 mg/kg IV Q4W for 120 weeks (maintenance)Placebo IV Q2W for 94 weeks, then Q4W for 120 weeksLecanemab 5 mg/kg IV Q4W for 4 weeks (titration), then 10 mg/kg IV Q4W for 208 weeksPlacebo IV Q4W for 216 weeks
Duration216 weeks216 weeks216 weeks216 weeks

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
A45 Trial: Change from baseline in PACC5 at 216 weeks; A3 Trial: Change from baseline in amyloid PET SUVr at 216 weeksPrimaryDesign paper - no resultsDesign paper - no results
A3 Trial key secondary: Tau PET (neocortical composite)SecondaryDesign paper - no resultsDesign paper - no results
Amyloid PET (A45 Trial secondary)SecondaryDesign paper - no resultsDesign paper - no results
Tau PET (A45 Trial secondary)SecondaryDesign paper - no resultsDesign paper - no results
Plasma biomarkers (Aβ42, Aβ40, Aβ42/40 ratio) - exploratorySecondaryDesign paper - no resultsDesign paper - no results
CSF biomarkers (p-tau-217, p-tau-181, total tau, Aβ42, Aβ40) - exploratorySecondaryDesign paper - no resultsDesign paper - no results
Neurodegeneration markers (CSF NFL, neurogranin) - exploratorySecondaryDesign paper - no resultsDesign paper - no results
NoteAdverseDesign paper - no safety data availableDesign paper - no safety data available

Subgroup Analysis

Design paper - subgroup analyses planned but not yet performed. Randomization stratified by APOE ε4 status and geographical region.


Criticisms

  • Design paper only - efficacy and safety results not yet available
  • Recruitment challenges anticipated, particularly identifying young asymptomatic individuals (age 55+) with elevated amyloid
  • COVID-19 pandemic expected to impact recruitment
  • Under-representation of diverse populations was an issue in prior A4 study and may be challenging to address
  • Long duration (216 weeks) may increase dropout rates

Funding

Public-Private Partnership: National Institute on Aging (National Institutes of Health) and Eisai Inc.; conducted through the Alzheimer's Clinical Trial Consortium (ACTC)

Based on: AHEAD 3–45 (Alzheimer's & Dementia, 2023)

Authors: Michael S. Rafii, Reisa A. Sperling, Michael C. Donohue, ..., Paul S. Aisen

Citation: Alzheimers Dement. 2023 April; 19(4): 1227-1233

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